Journal: bioRxiv

Article Title: Epithelial N-methyl-D-aspartate (NMDA) receptors mediate renal vasodilation by affecting kidney autoregulation

doi: 10.1101/2023.12.04.569973

Figure Lengend Snippet: NMDAR RNA expression along the nephron. The mandatory Grin 1 subunit is expressed in all the renal segments, especially in the medulla regions of the nephron. In the medulla region all NMDAR subunit are expressed, while in the cortex in addition to Grin1, Grin2D is the majority expressed in the distal nephron in addition to grin2C and Grin3A in DCT and in less magnitude in CNT and CCD.TMP: Transcript per millon. [PTS1] initial segment of proximal tubule; [PTS2] straight segment of proximal tubule; [PTS3] last segment of the proximal straight tubule in the outer stripe of outer medulla; [DTL1] short descending limb of the loop of Henle; [DTL2] long descending limb of the loop of Henle in the outer medulla; [DTL3] long descending limb of the loop of Henle in the inner medulla; [ATL]) thin ascending limb of the loop of Henle; [MTAL] medullary thick ascending limb of the loop of Henle; [CTAL] cortical thick ascending limb of the loop of Henle; [DCT] distal convoluted tubule; [CNT] connecting tubule, cortical collecting duct [CCD], outer medullary collecting duct [OMCD], and inner medullary collecting duct [IMCD])

Article Snippet: Tissue incubation with antibodies against GluN1 (StressMarq, S308-48) at 1:5000 dilution was performed overnight at 4°C.

Techniques: RNA Expression

Journal: Neural Regeneration Research

Article Title: Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation

doi: 10.4103/1673-5374.184494

Figure Lengend Snippet: Effect of MNS on NR1 expression in the prefrontal cortex of rats with TBI-induced coma (western blot assay). Control group: Sham-operated rats (skin incision but no TBI). TBI group: Weight-drop method was used to establish a model of TBI. Stimulated group: Comatose rats with TBI underwent MNS. Antagonist group: Comatose rats with TBI and given MNS received an intracerebroventricular injection of the OX1R antagonist SB334867. Data are expressed as the mean ± SD ( n = 6 rats per time point per group). * P < 0.05, vs . control group; # P < 0.05, vs . TBI group; † P < 0.05, vs . stimulated group; § P < 0.05, vs . 6 h; ‡ P < 0.05, vs . 12 h (one-way analysis of variance). MNS: Electrical stimulation of the median nerve; TBI: traumatic brain injury; h: hours.

Article Snippet: The membranes were incubated with rabbit anti-NR1 polyclonal antibody (1:200; Wuhan Boster Biotechnology Co., Ltd., Wuhan, Hubei Province, China) and mouse anti-β-actin monoclonal antibody (1:500; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China) overnight at 4°C, and washed in Tris buffered saline with Tween 20.

Techniques: Expressing, Western Blot, Control, Injection

Journal: Neural Regeneration Research

Article Title: Mechanisms responsible for the effect of median nerve electrical stimulation on traumatic brain injury-induced coma: orexin-A-mediated N-methyl-D-aspartate receptor subunit NR1 upregulation

doi: 10.4103/1673-5374.184494

Figure Lengend Snippet: Effect of MNS on NR1 immunoreactivity in the prefrontal cortex of rats with TBI-induced coma (× 400). Control group: Sham-operated rats (skin incision but no TBI). TBI group: Weight-drop method was used to establish a model of TBI. Stimulated group: Comatose rats with TBI underwent MNS. Antagonist group: Comatose rats with TBI and given MNS received an intracerebroventricular injection of the OX1R antagonist SB334867. Brown cells (red arrows) are NR1-immunoreactive. Positive immunostaining for NR1 was found in the cytoplasm, cell membrane, and nucleus of neurons in the prefrontal cortex. MNS: Electrical stimulation of the median nerve; TBI: traumatic brain injury; h: hours.

Article Snippet: The membranes were incubated with rabbit anti-NR1 polyclonal antibody (1:200; Wuhan Boster Biotechnology Co., Ltd., Wuhan, Hubei Province, China) and mouse anti-β-actin monoclonal antibody (1:500; Zhongshan Golden Bridge Biotechnology Co., Ltd., Beijing, China) overnight at 4°C, and washed in Tris buffered saline with Tween 20.

Techniques: Control, Injection, Immunostaining, Membrane

Journal: BMC Anesthesiology

Article Title: The interplay of BDNF-TrkB with NMDA receptor in propofol-induced cognition dysfunction

doi: 10.1186/s12871-018-0491-y

Figure Lengend Snippet: Immunohistochemical staining revealed that the protein levels of NMDAR and BDNF in hippocampus tissue. A. NMDAR and BDNF were expressed abundantly both in the nucleus and cytoplasm in the PND group, the improvement effect of the PNK group was inhibited compared to PN, the improvement effect of the PMD group was inhibited compared PD group. B. The mRNA expression of NMDAR, proBDNF, mBDNF, TrkB, ERK1 and CREB were tested by RT-PCR. The mRNA levels of NMDAR, mBDNF and TrkB were lower in the P group than those in the NS group, while the level of proBDNF was up-regulated in the P group compared to NS group. However, there were no obvious different expression of ERK1and CREB between the NS group and the others. C, F. The protein phosphorylation of NMDAR, proBDNF, mBDNF, TrkB, ERK1 and CREB were determined by Western blot. The phosphorylation levels of NMDAR, TrkB, ERK1 and CREB in the PND group were highest, while P group were the lowest. D, E. The ratio of p-NMDAR and NMDAR, the ratio of p-TrkB andTrkB. *versus NS group, P < 0.05; #versus P group, P < 0.05; &versus PN group, P < 0.05; $versus PD group, P < 0.05

Article Snippet: The protein expression and phosphorylation of NMDA receptor, proBDNF, mBDNF, TrkB, ERK1 and CREB were determined by Western blot using specific monoclonal antibodies as follows: rabbit anti-NMDAR (PA1222, BosterBio), rabbit anti-p-NMDAR (ABN99, Millipore), mouse anti-proBDNF (sc-65,514, Santa Cruz), rabbit anti-mBDNF (D160119, Sangon Biotech, China), rabbit anti-TrkB (ab18987, Abcam), rabbit anti-p-TrkB (ABN1381, Millipore), rabbit anti-CREB (ab32515, Abacm), rabbit anti-p-CREB (ab32096, Abcam).

Techniques: Immunohistochemical staining, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction, Phospho-proteomics, Western Blot

Journal: Molecular Neurobiology

Article Title: Neuroligin-3 Regulates Excitatory Synaptic Transmission and EPSP-Spike Coupling in the Dentate Gyrus In Vivo

doi: 10.1007/s12035-021-02663-9

Figure Lengend Snippet: Immunoblot data from hippocampal synaptosomes (males, 8–12 weeks, n = number of pairs)

Article Snippet: The following primary antibodies were used: Nlgn1 (RRID: AB_887747, Synaptic Systems, Göttingen, Germany), PSD-95 (RRID: AB_2877189, NeuroMAB, Davis, CA, USA), AMPA receptor subunit 1 (GluR1, RRID: AB_2113602, Chemicon, Temecula, CA, USA), AMPA receptor subunit 2 (GluR2, RRID: AB_2113732, Synaptic Systems, Göttingen, Germany), NMDA receptor subunit 1 (NR1, RRID: AB_887750, Synaptic Systems, Göttingen, Germany), vesicular glutamate transporter 1 (VGlut1, RRID: AB_887878, Synaptic Systems, Göttingen, Germany), vesicular inhibitory amino acid transporter (VIAAT, RRID: AB_2189938, Synaptic Systems, Göttingen, Germany), gephyrin (RRID: AB_887719, Synaptic Systems, Göttingen, Germany), actin (RRID: AB_258912, Sigma-Aldrich, St. Louis, MO, USA), and Nlgn2 (antibody 799, Nils Brose).

Techniques: Western Blot